OPTIKA

Laboratuvar Mikroskopları

B-290 Series

This series incorporates all the experience gathered by OPTIKA Microscopes in the field of light microscopy, adapted specifically for common laboratory applications. Suitable for routine microscopy with brightfield, darkfield, phase contrast and LED fluorescence, designed to last.

B-380 Series

This series incorporates all the experience gathered by OPTIKA Microscopes in the field of light microscopy, adapted specifically for common laboratory applications. Suitable for routine microscopy with brightfield, darkfield (oil and dry), phase contrast, fluorescence and polarized light, designed to be extremely stable on the bench and last long.

B-510 Series

OPTIKA B-510 Series represents the ideal, ultra-modern, advanced routine microscope for efficient analysis in transmitted light applications, carefully engineered considering all the relevant aspects for users. This series offers user-friendly operations, robustness, durability and superb resolution, delivering contrasted and sharp images through the impressive IOS W-PLAN optics, the Infinity Corrected Optics (IOS) providing the best cost-effective choice for high contrast and resolution, matching all the requirements of labs requiring good quality routinary optics and designed to ensure field flatness up to F.N. 22. The full Köhler system optimizes the microscope optical path to produce high sample contrast and homogeneous bright light, reducing image artifacts, whilst the state-of-the-art, exclusive X-LED3 lighting source  makes sure your specimen will be properly and homogeneously illuminated with incredibly low consumptions and significantly long lifespan. With multi-head observation systems, up to 5 people/colleagues can observe the same image on B-510; ideal for teaching and training students, especially in the medical field. The main observer and additional viewers will benefit of an extremely homogeneous light conditions, with a three-colour pointer with settable intensity to highlight points of interest.

B-810/B-1000 Series

OPTIKA Microscopes, thanks to the long experience achieved in microscopy development, has conceived the new B-810/B-1000: a major leap in our technological offer. As a flagship instrument, B-810 & B-1000 originates from customer most demanding feedbacks and needs. Its modularity and versatility will allow to find the perfect place in any clinical or basic reasearch laboratory. All controls are easily accessible and comfortable also for extended periods of observation.

IM-3 Series

Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g., a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. IM-3 Series includes a version for simultaneously brightfield and phase contrast method, engineered and designed to be your ideal solution for fast and reliable routine inspections. The glass stage surface allows an optimal visual access to the objective turret. A particularly simple and ingenious optical design allows stable alignments and smooth and accurate movements.

IM-5 Series

OPTIKA IM-5 is a new inverted research microscope producing brilliant images for the examination of living cells, organisms and several other specimens in large flasks, combining brightfield, darkfield and phase contrast techniques for the most demanding users. This inverted research microscope drives to new horizons providing Köhler condenser, ergonomic handy controls and significant unique features, such as the highest F.O.V. available on an inverted microscope (F.N. 24 mm). IM-5 is freely configurable in terms of objectives, by choosing among: – IOS LWD W-PLAN (Plan-Achromatic LWD for brightfield, F.N. 22) – IOS LWD W-PLAN PH (Plan-Achromatic LWD for phase contrast, F.N. 22) – IOS LWD U-PLAN F (Semi-Apochromatic LWD for brightfield, F.N. 25) – IOS LWD U-PLAN F PH (Semi-Apochromatic LWD for phase contrast, F.N. 25)

POL Series

Polarized light microscopy is an optical microscopy technique involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted or incident light can, optionally, be blocked with a polariser orientated at 90 degrees to the illumination. These illumination techniques are most commonly used on birefringent samples where the polarized light interacts strongly with the sample and so generating contrast with the background. Polarized light microscopy is used extensively in optical mineralogy. As polarised light passes through a birefringent sample, the phase difference between the fast and slow directions varies with the thickness, and wavelength of light used. The optical path difference (o.p.d.) is defined as o . p . d . = Δ n x t where t is the thickness of the sample. This then leads to a phase difference between the light passing in the two vibration directions of δ = 2 π ( Δ n x t / λ ) For example, if the optical path difference is λ / 2 , then the phase difference will be π , and so the polarisation will be perpendicular to the original, resulting in all of the light passing through the analyser for crossed polars. If the optical path difference is n x λ, then the phase difference will be 2 n x π , and so the polarisation will be parallel to the original. This means that no light will be able to pass through the analyser which it is now perpendicular to. The Michel-Levy Chart arises when polarised white light is passed through a birefringent sample. If the sample is of uniform thickness, then only one specific wavelength will meet the above condition described above, and be perpendicular to the direction of the analyser. This means that instead of polychromatic light being viewed at the analyser, one specific wavelength will have been removed. This information can be used in a number of ways: – If the birefringence is known, then the thickness, t, of the sample can be determined – If the thickness is known, then the birefringence of the sample can be determined As the order of the optical path difference increases, then it is more likely that more wavelengths of light will be removed from the spectrum. This results in the appearance of the colour being “washed out”, and it becomes more difficult to determine the properties of the sample. This, however, only occurs when the sample is relatively thick when compared to the wavelength of light.

FLUO Series

Epi Fluorescence microscopes A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances. The “fluorescence microscope” refers to any microscope that uses fluorescence to generate an image. The Epi Fluorescence microscope is equipped with a fluorescence illuminator wich generates incident fluorescence light.highlight points of interest. Principle The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (HBO mercury-vapor lamps are common; more advanced forms are high-power LEDs), the excitation filter, the dichroic mirror, and the emission filter. The filters and the dichroic mirror are chosen to match the spectral excitation and emission characteristics of the fluorophore used to label the specimen. In this manner, the distribution of a single fluorophore (color) is imaged at a time. Multi-color images of several types of fluorophores must be composed by combining several single-color images. Most fluorescence microscopes in use are epifluorescence microscopes, where excitation of the fluorophore and detection of the fluorescence are done through the same light path (through the objective). These microscopes are widely used in biology and are the basis for more advanced microscope designs. Epifluorescence microscopy The majority of fluorescence microscopes, especially those used in the life sciences, are of the epifluorescence design. Light of the excitation wavelength illuminates the specimen through the objective lens. The fluorescence emitted by the specimen is focused to the detector by the same objective that is used for the excitation which for greater resolution will need objective lens with higher numerical aperture. Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and the epifluorescence method therefore gives a high signal-to-noise ratio. The dichroic beamsplitter acts as a wavelength specific filter, transmitting fluoresced light through to the eyepiece or detector, but reflecting any remaining excitation light back towards the source.